Drug Discovery

Assay and Screening Platforms

Home Drug Discovery Assay and Screening Platforms

Our Assays and Screening approach to high throughput hit hunting benefits from the application of focused discovery subsets of the 1.5 million small molecules in ChemDiv's proprietary discovery inventory. These are subjected to well-defined assay and high and medium throughput screening platforms to identify and confirm compounds which serve as starting points for medicinal chemistry lead optimization.

ASSAY AND SCREENING PLATFORMS
  • Luminescence: Reporter genes; ELISAs; Complementation assays;
  • Fluorescence: FLINT, FRET, TR-FRET, FP, FLIPR; GFP; ELISAs
  • Radio-Isotope: Filter assays (ligand-binding/competition)
  • Absorbance: OD, ABS spectra
  • In vitro assays: Cell calcium, ELISA, Multiplex ELISA, Western blot, Kinase assays (DiscoveRx), cAMP assay (Perkin Elmer), Enzyme binding assay (FP), Cell cycle Apoptosis/Necrosis, ADME assays, CACO-2 and other membrane permeability assays
  • Cell Assays: cycle analysis, apoptosis, in vitro chemoinvasion
  • Molecular and Cell Biology: Expression / Reporter Constructs; Cell lines and primary cultures; Transient/stable transfection.
  • Custom Assay Development (Clients' assays, Low throughput/labor intensive assays: patch-clamp, histochemistry, etc.

 

AUTOMATED SCREENING PLATFORMS

Biomek 2000, NX, FX work stations with multimode 96/384-well plate readers; Microbeta PLUS 1450 (PerkinElmer), scintillation counters; Victor2V multimode 96/384/1536 plate readers; SpectraMax Plus 96/384 spectral plate readers; Harvesters Tomtec Mach III (Tomtec); 96 /384 well format Flow Cytometer.